For Your Education - FYE Glow in the Dark Water Experiment – For Your Education
For Your Education

Glow in the Dark Water Experiment

Key concepts

Chemistry
Water
Light
Fluorescence
Energy
Ultraviolet light

 

Background

Tonic water is a carbonated beverage that has a chemical called quinine dissolved in it. Quinine is made from the bark of a tree and has been used for centuries as a treatment for malaria. Quinine not only gives tonic water a characteristic bitter taste (which is offset today by the addition of sweeteners to bottled tonic water) but this chemical can also be very fluorescent under the right conditions.

Under an ultraviolet “black light,” the quinine in tonic water makes the water fluoresce a brilliant, bright blue (even though only a relatively small amount of quinine is dissolved in the water). In general, something fluoresces because it has absorbed light energy, which makes it excited, and then it releases (or emits) light as it returns to its normal, unexcited state. Part of why we find things that glow under ultraviolet lights—such as some minerals, fish and tonic water—to be fascinating is because we cannot see the (ultraviolet) light they absorb but can see the visible light they emit (which is blue in the case of quinine).

 

Materials

 

Preparation

 

Procedure

 

Observations and results

When you added a few drops of bleach to the tonic water, did it stop fluorescing under the ultraviolet black light?

You should have clearly seen that the tonic water glowed a brilliant, bright blue color when you put it under the ultraviolet black light (before adding bleach). This is because the (invisible) ultraviolet light from the black light is absorbed by the quinine in the tonic water, and this excites the quinine. When the quinine becomes unexcited, it releases visible blue light that we see. After adding and mixing in a few drops of bleach with the tonic water, however, it should have stopped glowing. Why? Bleach is an oxidizing agent and can disrupt and break certain chemical bonds (specifically carbon–carbon double bonds). It is in these chemical bonds that the quinine absorbs the ultraviolet light, so by adding bleach to the tonic water it makes the quinine unable to absorb ultraviolet light anymore, and so it can no longer emit blue light.

 

Courtesy of Science Buddies